Nestin Antibody

NSJ Bioreagents
Product Code: NSJ-F53752
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F53752-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Storage:
Aliquot the Nestin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human hepatocarcinoma tissue stained with the Nestin antibody~
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Immunofluorescence analysis of Nestin antibody and HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L)

IHC analysis of FFPE human hepatocarcinoma tissue stained with the Nestin antibody~
Immunofluorescence analysis of Nestin antibody and HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L)

Documents

Further Information

Application Details:
IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Format:
Purified
Formulation:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1560-1590 from the human protein was used as the immunogen for this Nestin antibody.
Limitation:
This Nestin antibody is available for research use only.
Purity:
Purified
Uniprot #:
P48681