HSP60 Antibody

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NSJ Bioreagents
Product Code: NSJ-R36413
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R36413-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store the HSP60 antibody at -20oC.

Images

1 / 5
Western blot testing of human A) heart, B) kidney, C) liver lysates and pig D) heart, E) kidney, F) liver lysates using HSP60 antibody at 0.03ug/ml. Predicted molecular weight: ~61kDa.
2 / 5
Western blot testing of rat kidney lysate using HSP60 antibody at 0.1ug/ml. Predicted molecular weight: ~61 kDa.
3 / 5
IHC staining of FFPE human kidney tissue with HSP60 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
4 / 5
IF/ICC staining of fixed and permeabilized human A431 cells with HSP60 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
5 / 5
IF/ICC staining of fixed and permeabilized human HeLa cells with HSP60 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
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Western blot testing of human A) heart, B) kidney, C) liver lysates and pig D) heart, E) kidney, F) liver lysates using HSP60 antibody at 0.03ug/ml. Predicted molecular weight: ~61kDa.
Western blot testing of rat kidney lysate using HSP60 antibody at 0.1ug/ml. Predicted molecular weight: ~61 kDa.
IHC staining of FFPE human kidney tissue with HSP60 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IF/ICC staining of fixed and permeabilized human A431 cells with HSP60 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
IF/ICC staining of fixed and permeabilized human HeLa cells with HSP60 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Documents

Further Information

Application Details:
Western blot: 0.01-0.03ug/ml,Immunohistochemistry (FFPE): 5ug/ml,Immunofluorescence (FFPE): 10 ug/ml,ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the HSP60 antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: HSPD1, Heat shock 60kDa protein 1, Chaperonin, GROEL
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
3329
Immunogen:
Amino acids NLEDVQPHDLGK were used as the immunogen for this HSP60 antibody.
Limitation:
This HSP60 antibody is available for research use only.
Purity:
Antigen affinity