GAPDH Loading Control Antibody

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NSJ Bioreagents
Product Code: NSJ-R34262
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34262-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the GAPDH loading control antibody at -20oC.

Images

1 / 5
Western blot testing of human 1) HEK293 and 2) HeLa cell lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
2 / 5
Western blot testing of human 1) liver and 2) tonsil lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
3 / 5
Western blot testing of 1) mouse liver and 2) rat brain lysate with GAPDH loading control antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
4 / 5
IHC testing of FFPE human kidney tissue with GAPDH loading control antibody at 5ug/ml (inset without primary Ab). Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
5 / 5
IF/ICC staining of human HeLa cells with Alexa Fluor 488 secondary, and GAPDH antibody at 5ug/ml (blue = DAPI nuclear counterstain).
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Western blot testing of human 1) HEK293 and 2) HeLa cell lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of human 1) liver and 2) tonsil lysate with GAPDH loading control antibody at 0.1ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of 1) mouse liver and 2) rat brain lysate with GAPDH loading control antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
IHC testing of FFPE human kidney tissue with GAPDH loading control antibody at 5ug/ml (inset without primary Ab). Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
IF/ICC staining of human HeLa cells with Alexa Fluor 488 secondary, and GAPDH antibody at 5ug/ml (blue = DAPI nuclear counterstain).

Documents

Further Information

Application Details:
Western blot: 0.1-0.5ug/ml,Immunofluorescence/Immunocytochemistry: 5ug/ml,Immunohistochemistry (FFPE): 5ug/ml,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the GAPDH loading control antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
2597
Immunogen:
Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody.
Limitation:
This GAPDH loading control antibody is available for research use only.
Purity:
Antigen affinity