GRP78 Antibody (BiP)

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NSJ Bioreagents
Product Code: NSJ-R36416
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R36416-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store the GRP78 antibody at -20oC.

Images

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Western blot testing of A) HepG2 and B) MCF7 cell lysate with GRP78 antibody at 0.1ug/ml. Predicted molecular weight: ~73kDa, but routinely observed at 70-78kDa.
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Western blot testing of 1) mouse liver and 2) mouse testis lysate with GRP78 antibody at 0.3ug/ml and 3) rat brain lysate with GRP78 antibody at 0.5ug/ml. Expected molecular weight: 70-78 kDa.
3 / 4
IF/ICC testing of fixed and permeabilized human HeLa cells with GRP78 antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human cortex tissue with GRP78 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of A) HepG2 and B) MCF7 cell lysate with GRP78 antibody at 0.1ug/ml. Predicted molecular weight: ~73kDa, but routinely observed at 70-78kDa.
Western blot testing of 1) mouse liver and 2) mouse testis lysate with GRP78 antibody at 0.3ug/ml and 3) rat brain lysate with GRP78 antibody at 0.5ug/ml. Expected molecular weight: 70-78 kDa.
IF/ICC testing of fixed and permeabilized human HeLa cells with GRP78 antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
IHC testing of FFPE human cortex tissue with GRP78 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 1-3ug/ml,Immunofluorescence/Immunocytochemistry: 5ug/ml,Immunohistochemistry (FFPE): 5ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the GRP78 antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: HSPA5; Heat shock 70kDa protein 5; glucose-regulated protein 78
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
3309
Immunogen:
Amino acids ESHQDADIEDFK were used as the immunogen for this GRP78 antibody.
Limitation:
This GRP78 antibody is available for research use only.
Purity:
Antigen affinity