AKT3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R33341
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R33341-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store the AKT3 antibody at -20oC.

Images

1 / 4
Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
2 / 4
Western blot of HEK293 lysate overexpressing AKT3 probed with AKT3 antibody (mock transfection on right). Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
3 / 4
IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
4 / 4
IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
Western blot of HEK293 lysate overexpressing AKT3 probed with AKT3 antibody (mock transfection on right). Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.
IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 1-3ug/ml,Immunohistochemistry (FFPE): 5ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the AKT3 antibody should be determined by the researcher.

1. This AKT3 antibody does not react with human AKT1/2.

Description:
Additional name(s) for this target protein: Protein kinase Akt-3; RAC-PK-gamma
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
10000
Immunogen:
Amino acids CSPTSQIDNIGEEEM were used as the immunogen for this AKT3 antibody. (1)
Limitation:
This AKT3 antibody is available for research use only.
Purity:
Antigen affinity