Sprouty Antibody

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NSJ Bioreagents
Product Code: NSJ-R33305
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R33305-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the Sprouty antibody at -20oC.

Images

1 / 4
Western blot testing of human kidney lysate with Sprouty antibody at 0.3ug/ml. Predicted molecular weight ~35 kDa.
2 / 4
FACS testing of fixed and permeabilized human HEK293 cells with Sprouty antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
3 / 4
IF/ICC testing of fixed and permeabilized human HeLa cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
4 / 4
IF/ICC testing of fixed and permeabilized human HepG2 cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
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Western blot testing of human kidney lysate with Sprouty antibody at 0.3ug/ml. Predicted molecular weight ~35 kDa.
FACS testing of fixed and permeabilized human HEK293 cells with Sprouty antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
IF/ICC testing of fixed and permeabilized human HeLa cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
IF/ICC testing of fixed and permeabilized human HepG2 cells with Sprouty antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Documents

Further Information

Application Details:
Western blot: 1-3ug/ml (2),Immunohistochemistry (FFPE): 1-3ug/ml (1),Immunofluorescence/Immunocytochemistry: 10ug/ml,FACS: 10ug/million cells in 0.2ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the Sprouty antibody should be determined by the researcher.

1. Reported by customer to detect Sprouty (nuclear staining) in FFPE mouse brain. 2. Reported by customer to detect Sprouty in embryonal rhabdomyosarcoma (ERMS) lysate.

Description:
Additional name(s) for this target protein: SPRY1
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
10252
Immunogen:
Amino acids CPSRGQGKPS were used as the immunogen for this Sprouty antibody.
Limitation:
This Sprouty antibody is available for research use only.
Purity:
Antigen affinity