PRAK Antibody

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NSJ Bioreagents
Product Code: NSJ-R35126
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R35126-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store the PRAK antibody at -20oC.

Images

1 / 3
Western blot of HEK293 lysate overexpressing mouse MK5/PRAK (first lane) or mouse MK2 (second lane) tested with right ) PRAK antibody (<a href=../tds/prak-antibody-r36080>cat # R36080</a>, 0.5ug/ml) and left) PRAK antibody (cat # R35126, 0.5ug/ml). No cross-reaction seen with MK2 protein.
2 / 3
PRAK antibody (<a href=../tds/prak-antibody-r36080>cat # R36080</a> and R35126) used at 1.5ug for immunoprecipitation from lysates of PRAK/ERK3 double knockout mouse embryonic firbroblasts, with (third and fifth lanes) and without (fourth and sixth lanes) rescued PRAK/ERK3 expression through retroviral transduction.
3 / 3
IHC staining of FFPE human kidney with PRAK antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
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Western blot of HEK293 lysate overexpressing mouse MK5/PRAK (first lane) or mouse MK2 (second lane) tested with right ) PRAK antibody (<a href=../tds/prak-antibody-r36080>cat # R36080</a>, 0.5ug/ml) and left) PRAK antibody (cat # R35126, 0.5ug/ml). No cross-reaction seen with MK2 protein.
PRAK antibody (<a href=../tds/prak-antibody-r36080>cat # R36080</a> and R35126) used at 1.5ug for immunoprecipitation from lysates of PRAK/ERK3 double knockout mouse embryonic firbroblasts, with (third and fifth lanes) and without (fourth and sixth lanes) rescued PRAK/ERK3 expression through retroviral transduction.
IHC staining of FFPE human kidney with PRAK antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.5-1.5ug/ml ,Immunohistochemistry (FFPE): 4-8ug/ml,Immunoprecipitation: suitable,ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the PRAK antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: P38-regulated/activated protein kinase, Mitogen-activated protein kinase-activated protein kinase 5, MK5
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
8550
Immunogen:
Amino acids PQVLEAQRRHQKEKS were used as the immunogen for this PRAK antibody.
Limitation:
This PRAK antibody is available for research use only.
Purity:
Antigen affinity