NONO Antibody / p54nrb

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NSJ Bioreagents
Product Code: NSJ-R34895
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34895-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store the NONO antibody at -20oC.

Images

1 / 4
IHC staining of FFPE human spleen using NONO antibody at 3.8ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
2 / 4
Western blot testing of mouse brain lysate with NONO antibody at 0.1ug/ml. Predicted molecular weight: ~54kDa.
3 / 4
Western blot of HEK293 lysate overexpressing human NONO probed with NONO antibody (mock transfection in lane 1). Predicted molecular weight: ~54kDa.
4 / 4
IHC staining of FFPE human testis using NONO antibody at 3.8ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
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IHC staining of FFPE human spleen using NONO antibody at 3.8ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
Western blot testing of mouse brain lysate with NONO antibody at 0.1ug/ml. Predicted molecular weight: ~54kDa.
Western blot of HEK293 lysate overexpressing human NONO probed with NONO antibody (mock transfection in lane 1). Predicted molecular weight: ~54kDa.
IHC staining of FFPE human testis using NONO antibody at 3.8ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.1-0.3ug/ml,Immunohistochemistry (FFPE): 3-6ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the NONO antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: P54NRB, Non-POU domain containing octamer-binding
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
4841
Immunogen:
Amino acids NRAAPGAEFAPNK were used as the immunogen for this NONO antibody.
Limitation:
This NONO antibody is available for research use only.
Purity:
Antigen affinity