AP2A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R33860
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R33860-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the AP2A1 antibody at -20oC.

Images

1 / 4
Western blot testing of human frontal cortex lysate with AP2A1 antibody at 0.01ug/ml. Predicted molecular weight: ~108kDa.
2 / 4
Western blot testing of mouse brain lysate with AP2A1 antibody at 0.1ug/ml. The left panel shows the blot stained with Ponceau red for total protein (~5ug/lane).
3 / 4
ICC staining of methanol-fixed HeLa cells transfected with mouse Ap2a1 using AP2A1 antibody at 0.1ug/ml.
4 / 4
IHC testing of FFPE human brain (cortex) with AP2A1 antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot testing of human frontal cortex lysate with AP2A1 antibody at 0.01ug/ml. Predicted molecular weight: ~108kDa.
Western blot testing of mouse brain lysate with AP2A1 antibody at 0.1ug/ml. The left panel shows the blot stained with Ponceau red for total protein (~5ug/lane).
ICC staining of methanol-fixed HeLa cells transfected with mouse Ap2a1 using AP2A1 antibody at 0.1ug/ml.
IHC testing of FFPE human brain (cortex) with AP2A1 antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.01-0.03ug/ml,Immunocytochemistry: suitable,Immunohistochemistry (FFPE): 5ug/ml,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the AP2A1 antibody should be determined by the researcher.

1. This AP2A1 antibody is specific for isoform 1 of the protein.

Description:
Additional name(s) for this target protein: Adaptor-related protein complex 2, alpha 1 subunit
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
160
Immunogen:
Amino acids ELEPPAPESPMALLADPAPAAD were used as the immunogen for this AP2A1 antibody.
Limitation:
This AP2A1 antibody is available for research use only.
Purity:
Antigen affinity