Pyruvate Carboxylase Antibody

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NSJ Bioreagents
Product Code: NSJ-R34431
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34431-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the Pyruvate Carboxylase antibody at -20oC.

Images

1 / 3
Western blot testing of mouse liver lysate with Pyruvate Carboxylase antibody at 0.03ug/ml. Predicted molecular weight: ~130kDa, observed here at 140~150kDa.
2 / 3
Western blot of HEK293 lysate overexpressing human Pyruvate Carboxylase probed with Pyruvate Carboxylase antibody (mock transfection in lane 1). Predicted molecular weight: ~130kDa, observed here at 140~150kDa.
3 / 3
IHC testing of FFPE human liver tissue with Pyruvate Caboxylase antibody at 5ug/ml. HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of mouse liver lysate with Pyruvate Carboxylase antibody at 0.03ug/ml. Predicted molecular weight: ~130kDa, observed here at 140~150kDa.
Western blot of HEK293 lysate overexpressing human Pyruvate Carboxylase probed with Pyruvate Carboxylase antibody (mock transfection in lane 1). Predicted molecular weight: ~130kDa, observed here at 140~150kDa.
IHC testing of FFPE human liver tissue with Pyruvate Caboxylase antibody at 5ug/ml. HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.03-0.1ug/ml,Immunohistochemistry (FFPE): 5ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the Pyruvate Carboxylase antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: PCB; PC
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
5091
Immunogen:
Amino acids KFKEVKKAYVEANQ were used as the immunogen for this Pyruvate Carboxylase antibody.
Limitation:
This Pyruvate Carboxylase antibody is available for research use only.
Purity:
Antigen affinity