HNF4A Antibody

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NSJ Bioreagents
Product Code: NSJ-R35678
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R35678-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the HNF4A antibody at -20oC.

Images

1 / 3
Western blot testing of HepG2 lysate with HNF4A antibody at 0.1ug/ml. Predicted molecular weight ~50/51/45kDa (isoforms 1/2/3).
2 / 3
IHC testing of FFPE human small intestine tissue with HNF4A antibody at 2ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
3 / 3
Western blot testing of mouse small intestine lysate with HNF4A antibody at 0.5ug/ml. Predicted molecular weight ~53/51.5 kDa (long/short form).
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Western blot testing of HepG2 lysate with HNF4A antibody at 0.1ug/ml. Predicted molecular weight ~50/51/45kDa (isoforms 1/2/3).
IHC testing of FFPE human small intestine tissue with HNF4A antibody at 2ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
Western blot testing of mouse small intestine lysate with HNF4A antibody at 0.5ug/ml. Predicted molecular weight ~53/51.5 kDa (long/short form).

Documents

Further Information

Application Details:
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 2ug/ml,ELISA (peptide) LOD: 1:32000
Application Note:
Optimal dilution of the HNF4A antibody should be determined by the researcher.

1. This HNF4A antibody will detect isoform a/b/c.

Description:
Additional name(s) for this target protein: Hepatic nuclear factor 4 alpha
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
3172
Immunogen:
Amino acids RLSKTLVDMDMADY were used as the immunogen for this HNF4A antibody.
Limitation:
This HNF4A antibody is available for research use only.
Purity:
Antigen affinity