MAPKAPK2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R36061
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R36061-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store the MAPKAPK2 antibody at -20oC.

Images

1 / 3
Western blot testing of mouse embryonic fibroblasat lysates from double KO mice (lanes 2 & 3) and rescued by introduction of MK2 gene in second lane. MK2 / MAPKAPK2 antibody was used at 0.5ug/ml. Predicted molecular weight: ~45kDa.
2 / 3
Western blot of HEK293 lysate overexpressing mouse MK2-GFP or MK5-GF probed with MAPKAPK2 antibody at 0.5ug/ml: cat # R36061 on left and <a href=../tds/mapkapk2-antibody-r35113>cat # R35113</a> on right. Predicted molecular weight of MAPKAPK2+GFP: ~72kDa.
3 / 3
Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and <a href=../tds/mapkapk2-antibody-r35113>cat # R35113</a>, 1.5ug used) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.
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Western blot testing of mouse embryonic fibroblasat lysates from double KO mice (lanes 2 & 3) and rescued by introduction of MK2 gene in second lane. MK2 / MAPKAPK2 antibody was used at 0.5ug/ml. Predicted molecular weight: ~45kDa.
Western blot of HEK293 lysate overexpressing mouse MK2-GFP or MK5-GF probed with MAPKAPK2 antibody at 0.5ug/ml: cat # R36061 on left and <a href=../tds/mapkapk2-antibody-r35113>cat # R35113</a> on right. Predicted molecular weight of MAPKAPK2+GFP: ~72kDa.
Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and <a href=../tds/mapkapk2-antibody-r35113>cat # R35113</a>, 1.5ug used) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.

Documents

Further Information

Application Details:
Western blot: 0.5-1.5ug/ml,Immunoprecipitation: 1.5ug,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the MAPKAPK2 antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Mitogen-activated protein kinase-activated protein kinase 2; MK2
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
9261
Immunogen:
Amino acids SRVLKEDKERWED were used as the immunogen for this MAPKAPK2 antibody.
Limitation:
This MAPKAPK2 antibody is available for research use only.
Purity:
Antigen affinity