GADD34 Antibody

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NSJ Bioreagents
Product Code: NSJ-R33103
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R33103-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store the GADD34 antibody at -20oC.

Images

1 / 5
Western blot testing of 1) HEPG2 cell lysate and 2) KLY negative control cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73 kDa.
2 / 5
IHC staining of FFPE human liver with GADD34 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
3 / 5
ICC/IF testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
4 / 5
ICC/IF testing of fixed and permeabilized human HeLa cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
5 / 5
FACS testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
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Western blot testing of 1) HEPG2 cell lysate and 2) KLY negative control cell lysate with GADD34 antibody at 0.1ug/ml. Predicted molecular weight ~73 kDa.
IHC staining of FFPE human liver with GADD34 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
ICC/IF testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
ICC/IF testing of fixed and permeabilized human HeLa cells with GADD34 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
FACS testing of fixed and permeabilized human HepG2 cells with GADD34 antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).

Documents

Further Information

Application Details:
Western blot: 0.1-0.3ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml,ICC/Immunofluorescence: 10ug/ml,FACS: 10ug/million cells,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the GADD34 antibody should be determined by the researcher.

1. Not suitable for ICC/IF testing

Description:
Additional name(s) for this target protein: Growth arrest and DNA-damage-inducible 34; PPP1R15A
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
23645
Immunogen:
Amino acids AAALDLSGRRG were used as the immunogen for this GADD34 antibody.
Limitation:
This GADD34 antibody is available for research use only.
Purity:
Antigen affinity