DAP3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R34882
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34882-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the DAP3 antibody at -20oC.

Images

1 / 7
Western blot testing of HeLa lysate with DAP3 antibody at 3ug/ml. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
2 / 7
Western blot of HEK293 lysate overexpressing DAP3 probed with DAP3 antibody (mock transfection in lane 1). Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
3 / 7
Western blot of HeLa lysate (control in left lane, post si-RNA-mediated DAP3 knock-down expresson in right lane) with DAP3 antibody at 1ug/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
4 / 7
ICC staining of guanidinium thiocyanate-treated HeLa cells before (left) and after (right) si-RNA-mediated DAP3 knock-down using DAP3 antibody at 3ug/ml. Detection by DyLight 488 (green); nuclear DAPI stain (blue).
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Immunofluorescent staining of human MCF7 cells with DAP3 antibody at 5ug/ml.
6 / 7
IHC testing of FFPE human brain cortex tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
7 / 7
IHC testing of FFPE human tonsil tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of HeLa lysate with DAP3 antibody at 3ug/ml. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
Western blot of HEK293 lysate overexpressing DAP3 probed with DAP3 antibody (mock transfection in lane 1). Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
Western blot of HeLa lysate (control in left lane, post si-RNA-mediated DAP3 knock-down expresson in right lane) with DAP3 antibody at 1ug/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
ICC staining of guanidinium thiocyanate-treated HeLa cells before (left) and after (right) si-RNA-mediated DAP3 knock-down using DAP3 antibody at 3ug/ml. Detection by DyLight 488 (green); nuclear DAPI stain (blue).
Immunofluorescent staining of human MCF7 cells with DAP3 antibody at 5ug/ml.
IHC testing of FFPE human brain cortex tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human tonsil tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 3-6ug/ml,Immunohistochemistry (FFPE): 2.5ug/ml,Immunofluorescence/Immunocytochemistry: 1-5ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the DAP3 antibody should be determined by the researcher.

1. This DAP3 antibody will detect isoforms 1, 2 and 3.

Description:
Additional name(s) for this target protein: Death associated protein 3
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
7818
Immunogen:
Amino acids NPSLLERHCAYL were used as the immunogen for this DAP3 antibody. This sequence is common to isoforms 1, 2 and 3.
Limitation:
This DAP3 antibody is available for research use only.
Purity:
Antigen affinity
Uniprot #:
P51398