PDE4D Antibody

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NSJ Bioreagents
Product Code: NSJ-R35386
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R35386-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme Immunoassay (EIA)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the PDE4D antibody at -20oC.

Images

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Western blot testing of COS cell lysates with PDE4D antibody at 1ug/ml: 1) transfected with human PDE4D1 (~91kDa), 2) transfected with human PDE4D3 (~85kDa), 3) untransfected.
2 / 3
IHC testing of FFPE human heart tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
3 / 3
IHC testing of FFPE human skeletal muscle tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of COS cell lysates with PDE4D antibody at 1ug/ml: 1) transfected with human PDE4D1 (~91kDa), 2) transfected with human PDE4D3 (~85kDa), 3) untransfected.
IHC testing of FFPE human heart tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human skeletal muscle tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 1-3ug/ml,Immunohistochemistry (FFPE): 5ug/ml,Enzyme ImmunoAssay: binds recombinant protein,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the PDE4D antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Phosphodiesterase 4D
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
5144
Immunogen:
Amino acids QPEACVIDDRSPDT were used as the immunogen for this PDE4D antibody. This sequence is common to PDE4D1 (~91kDa), PDE4D2 (~77kDa) and PDE4D3 (~85kDa).
Limitation:
This PDE4D antibody is available for research use only.
Purity:
Antigen affinity