MIF Antibody

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NSJ Bioreagents
Product Code: NSJ-R34757
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34757-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme Immunoassay (EIA)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the MIF antibody at -20oC.

Images

1 / 4
Western blot testing of human thymus lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
2 / 4
Sandwich ELISA using the MIF antibody at 1.5ug/ml as the detect.
3 / 4
Western blot of Daudi (A) and Jurkat (B) lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
4 / 4
IHC testing of FFPE human thymus tissue with MIF antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of human thymus lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
Sandwich ELISA using the MIF antibody at 1.5ug/ml as the detect.
Western blot of Daudi (A) and Jurkat (B) lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
IHC testing of FFPE human thymus tissue with MIF antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.01-0.03ug/ml,Immunohistochemistry (FFPE): 5ug/ml,Enzyme ImmunoAssay: used as capture for recombinant protein (coat at 1-3ug/ml),ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the MIF antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Macrophage migration inhibitory factor
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
4282
Immunogen:
Amino acids NAANVGWNNSTFA were used as the immunogen for this MIF antibody.
Limitation:
This MIF antibody is available for research use only.
Purity:
Antigen affinity