FH Antibody

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NSJ Bioreagents
Product Code: NSJ-R34252
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34252-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme Immunoassay (EIA)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the FH antibody at -20oC.

Images

1 / 5
Western blot testing of human kidney lysate with FH antibody at 0.01ug/ml. Predicted molecular weight: ~55/50kDa (isoforms 1/2).
2 / 5
Western blot of HEK293 lysate overexpressing Fumarase probed with FH antibody (mock transfection in lane 1). Predicted molecular weight: ~55/50kDa (isoforms 1/2).
3 / 5
Sandwich ELISA using the FH antibody at 1.5ug/ml as the detect.
4 / 5
IHC testing of FFPE human skeletal muscle tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
5 / 5
IHC testing of FFPE human heart tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of human kidney lysate with FH antibody at 0.01ug/ml. Predicted molecular weight: ~55/50kDa (isoforms 1/2).
Western blot of HEK293 lysate overexpressing Fumarase probed with FH antibody (mock transfection in lane 1). Predicted molecular weight: ~55/50kDa (isoforms 1/2).
Sandwich ELISA using the FH antibody at 1.5ug/ml as the detect.
IHC testing of FFPE human skeletal muscle tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human heart tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.01-0.1ug/ml,Immunohistochemistry (FFPE): 2.5ug/ml,Enzyme ImmunoAssay: used as capture for recombinant protein (coat at 1-2ug/ml),ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the FH antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Fumarate hydratase, Fumarase
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
2271
Immunogen:
Amino acids HPNDHVNKSQSSND were used as the immunogen for this FH antibody. This sequence is common to isoforms 1 and 2.
Limitation:
This FH antibody is available for research use only.
Purity:
Antigen affinity
Uniprot #:
P07954