COMT Antibody

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NSJ Bioreagents
Product Code: NSJ-R35218
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R35218-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme Immunoassay (EIA)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the COMT antibody at -20oC.

Images

1 / 5
Western blot testing of human testis lysate with COMT antibody at 0.3ug/ml. Predicted molecular weight: ~30/25kDa (isoforms 1/2).
2 / 5
Sandwich ELISA using the COMT antibody at 0.5ug/ml as the detect.
3 / 5
IHC testing of FFPE human cerebellum with COMT antibody at 4ug/ml. HIER: steamed with pH9 Tris/EDTA, HRP-staining.
4 / 5
IHC testing of FFPE human testis tissue with COMT antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
5 / 5
IHC testing of FFPE human small intestine tissue with COMT antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
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Western blot testing of human testis lysate with COMT antibody at 0.3ug/ml. Predicted molecular weight: ~30/25kDa (isoforms 1/2).
Sandwich ELISA using the COMT antibody at 0.5ug/ml as the detect.
IHC testing of FFPE human cerebellum with COMT antibody at 4ug/ml. HIER: steamed with pH9 Tris/EDTA, HRP-staining.
IHC testing of FFPE human testis tissue with COMT antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human small intestine tissue with COMT antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Documents

Further Information

Application Details:
Western blot: 0.3-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml,Enzyme ImmunoAssay: binds recombinant protein (use at 0.5-2ug/ml),ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the COMT antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Catechol-O-methyltransferase
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
1312
Immunogen:
Amino acids QDIIPQLKKKYDVD were used as the immunogen for this COMT antibody.
Limitation:
This COMT antibody is available for research use only.
Purity:
Antigen affinity