AIRE Antibody

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NSJ Bioreagents
Product Code: NSJ-R35463
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R35463-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store the AIRE antibody at -20oC.

Images

1 / 3
Western blot testing of human lymph node lysate with AIRE antibody at 0.5ug/ml. Predicted molecular weight: ~58/37kDa (isoforms 1/2). Both observed bands are both blocked by the immunizing peptide.
2 / 3
IHC staining of FFPE human thymus with AIRE antibody at 2ug/ml. HIER: steamed with pH9 Tris/EDTA buffer, HRP-staining.
3 / 3
Western blot testing of untransfected (lane 1) and transfected (lane 2) HEK293 cell lysates with AIRE antibody at 0.05ug/ml.
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Western blot testing of human lymph node lysate with AIRE antibody at 0.5ug/ml. Predicted molecular weight: ~58/37kDa (isoforms 1/2). Both observed bands are both blocked by the immunizing peptide.
IHC staining of FFPE human thymus with AIRE antibody at 2ug/ml. HIER: steamed with pH9 Tris/EDTA buffer, HRP-staining.
Western blot testing of untransfected (lane 1) and transfected (lane 2) HEK293 cell lysates with AIRE antibody at 0.05ug/ml.

Documents

Further Information

Application Details:
Western blot: 0.5-1.5ug/ml,Immunohistochemistry (FFPE): 2.5-5ug/ml,ELISA (peptide) LOD: 1:128000
Application Note:
Optimal dilution of the AIRE antibody should be determined by the researcher.

1. This AIRE antibody is specific for isoforms 1 and 2.

Description:
Additional name(s) for this target protein: Autoimmune regulator, APECED, Automimmune polyendocrinopathy candidiasis ectodermal dystrophy
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
326
Immunogen:
Amino acids QSMARPAAPFPS were used as the immunogen for this AIRE antibody.
Limitation:
This AIRE antibody is available for research use only.
Purity:
Antigen affinity
Uniprot #:
O43918