PLK4 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R33184
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R33184-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store the PLK4 antibody at -20oC.

Images

1 / 4
Western blot testing of human colon lysate with PLK4 antibody at 2ug/ml. Expected molecular weight ~110kDa.
2 / 4
Immunofluorescence staining of paraformaldehyde fixed human A431 cells, permeabilized with 0.15% Triton, using PLK4 antibody.
3 / 4
Immunofluorescence staining of paraformaldehyde fixed human HeLa cells, permeabilized with 0.15% Triton, using PLK4 antibody.
4 / 4
FACS testing of fixed and permeabilized human HeLa cells with PLK4 antibody (blue) at 10ug/10^6 cells in 0.2ml and naive goat Ig (black).
❮❯

Western blot testing of human colon lysate with PLK4 antibody at 2ug/ml. Expected molecular weight ~110kDa.
Immunofluorescence staining of paraformaldehyde fixed human A431 cells, permeabilized with 0.15% Triton, using PLK4 antibody.
Immunofluorescence staining of paraformaldehyde fixed human HeLa cells, permeabilized with 0.15% Triton, using PLK4 antibody.
FACS testing of fixed and permeabilized human HeLa cells with PLK4 antibody (blue) at 10ug/10^6 cells in 0.2ml and naive goat Ig (black).

Documents

Further Information

Application Details:
Western blot: 1-3ug/ml,ELISA (peptide) LOD: 1:32000,Immunofluorescence: 10ug/ml,Flow cytometry: 10ug/million cells
Application Note:
Optimal dilution of the PLK4 antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Polo-like kinase 4
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
10733
Immunogen:
Amino acids ATCIGEKIEDFKVGN were used as the immunogen for this PLK4 antibody.
Limitation:
This PLK4 antibody is available for research use only.
Purity:
Antigen affinity