NCAM Antibody (isoform 2)

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NSJ Bioreagents
Product Code: NSJ-R34362
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34362-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store the NCAM antibody at -20oC.

Images

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IHC testing of FFPE human adrenal gland tissue with NCAM antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
2 / 3
Western blot testing of human frontal cortex lysate with NCAM antibody at 0.01ug/ml. The glycosylated form is detected with a molecular weight of ~180kDa.
3 / 3
FACS testing of fixed and permeabilized human U-251 cells with NCAM antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).
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IHC testing of FFPE human adrenal gland tissue with NCAM antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
Western blot testing of human frontal cortex lysate with NCAM antibody at 0.01ug/ml. The glycosylated form is detected with a molecular weight of ~180kDa.
FACS testing of fixed and permeabilized human U-251 cells with NCAM antibody (blue) at 10ug/10^6 cells and naive goat Ig (black).

Documents

Further Information

Application Details:
Western blot: 0.01-0.03ug/ml,Immunohistochemistry (FFPE): 5ug/ml,Flow cytometry: 10ug/million cells,ELISA (peptide) LOD: 1:32000
Application Note:
Optimal dilution of the NCAM antibody should be determined by the researcher.

1. This NCAM antibody is specific to isoform 2.

Description:
Additional name(s) for this target protein: Neural cell adhesion molecule 1, CD56; NCAM1
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
4684
Immunogen:
Amino acids KASWTRPEKQETLD were used as the immunogen for this NCAM antibody.
Limitation:
This NCAM antibody is available for research use only.
Purity:
Antigen affinity