EWS Antibody

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NSJ Bioreagents
Product Code: NSJ-R36298
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R36298-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store the EWS antibody at -20oC.

Images

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Western blot testing of nuclear HeLa lysate with EWS antibody at 0.3ug/ml. Predicted molecular weight: ~69kDa, but routinely observed at ~90kDa.
2 / 3
IHC staining of FFPE human kidney tissue with EWS antibody at 7ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
3 / 3
Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
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Western blot testing of nuclear HeLa lysate with EWS antibody at 0.3ug/ml. Predicted molecular weight: ~69kDa, but routinely observed at ~90kDa.
IHC staining of FFPE human kidney tissue with EWS antibody at 7ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.

Documents

Further Information

Application Details:
Western blot: 0.3-1ug/ml,Immunohistochemistry (FFPE): 7ug/ml,ELISA (peptide) LOD: 1:64000
Application Note:
Optimal dilution of the EWS antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Ewing sarcoma breakpoint region 1; EWSR1
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
2130
Immunogen:
Amino acids TSYDQSSYSQQNTYG were used as the immunogen for this EWS antibody.
Limitation:
This EWS antibody is available for research use only.
Purity:
Antigen affinity