ROBO1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R34076
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R34076-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot and store the ROBO1 antibody at -20oC.

Images

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IHC staining of FFPE human kidney tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
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Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
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IHC staining of FFPE human cortex tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
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Negative control: IHC staining of FFPE human cortex tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
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IF/ICC staining of fixed and permeabilized human U-2 OS cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
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IF/ICC staining of fixed and permeabilized human HeLa cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
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IHC staining of FFPE human kidney tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
IHC staining of FFPE human cortex tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
Negative control: IHC staining of FFPE human cortex tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
IF/ICC staining of fixed and permeabilized human U-2 OS cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
IF/ICC staining of fixed and permeabilized human HeLa cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Documents

Further Information

Application Details:
Immunofluorescence (FFPE): 10ug/ml,Immunohistochemistry (FFPE): 5-6ug/ml,ELISA (peptide) LOD: 1:16000
Application Note:
Optimal dilution of the ROBO1 antibody should be determined by the researcher.
Description:
Additional name(s) for this target protein: Roundabout 1
Format:
Antigen affinity purified
Formulation:
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Gene ID #:
6091
Immunogen:
Amino acids GDVDLSNKINEMK were used as the immunogen for this ROBO1 antibody.
Limitation:
This ROBO1 antibody is available for research use only.
Purity:
Antigen affinity