EpCAM Antibody

NSJ Bioreagents
Product Code: NSJ-V2184PE
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2184PE-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: VU-1D9
Regulatory Status: RUO
Target Species: Human
Application: Fluorescence-activated cell sorting (FACS)
Storage:
Store the EpCAM antibody at 2-8°C. Conjugate is light-sensitive protect from light.

Images

1 / 1
Surface FACS staining of HT29 cells using PE conjugated EpCAM antibody (red) and isotype control antibody (green).

Surface FACS staining of HT29 cells using PE conjugated EpCAM antibody (red) and isotype control antibody (green).

Documents

Further Information

Application Details:
FACS: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence: 1:50-1:100
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Description:
Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
PE Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
4072 (Human)
Immunogen:
Small cell lung carcinoma cells were used as the immunogen for this antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography