EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V2184SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2184SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: VU-1D9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE colon carcinoma stained with EpCAM antibody (clone VU-1D9).
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FACS staining (surface) of HT29 cells using EpCAM antibody (VU-1D9, red) and isotype control (green).
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Flow cytometry testing of PFA-fixed human MCF7 cells with EpCAM antibody (clone VU-1D9); Red=isotype control, Blue= CF488-labeled EpCAM antibody.
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Flow cytometry testing of bead-bound exosomes derived from human MCF-7 cells with (orange) and without (gray) CF555-labeled EpCAM antibody (clone VU-1D9).
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Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone VU-1D9, green) and Nucspot nuclear stain (red).
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SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone VU-1D9) as confirmation of integrity and purity.
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IHC testing of FFPE colon carcinoma stained with EpCAM antibody (clone VU-1D9).
FACS staining (surface) of HT29 cells using EpCAM antibody (VU-1D9, red) and isotype control (green).
Flow cytometry testing of PFA-fixed human MCF7 cells with EpCAM antibody (clone VU-1D9); Red=isotype control, Blue= CF488-labeled EpCAM antibody.
Flow cytometry testing of bead-bound exosomes derived from human MCF-7 cells with (orange) and without (gray) CF555-labeled EpCAM antibody (clone VU-1D9).
Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone VU-1D9, green) and Nucspot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone VU-1D9) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EpCAM antibody to be titered up or down for optimal performance.

1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. View the recombinant version of this EpCAM antibody.

Description:
Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. The VU-1D9 antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
4072
Immunogen:
Small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody VU-1D9.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography