Villin Antibody
| Code | Size | Price |
|---|
| NSJ-V3357SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: VIL1/1314
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Titration of the Villin antibody may be required for optimal performance.
Description:
Recognizes a protein of ~93kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
Limitation:
This Villin antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P09327