Ubiquitin Antibody

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NSJ Bioreagents
Product Code: NSJ-V3933SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3933SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: UBB/1748
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Ubiquitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.
2 / 3
IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/1748) as confirmation of integrity and purity.
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Western blot testing of human Raji cell lysate with Ubiquitin antibody (clone UBB/1748). Expected molecular weight ~9 kDa.
IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/1748). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free Ubiquitin antibody (clone UBB/1748) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Ubiquitin antibody should be determined by the researcher.
Description:
Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.
Limitation:
This Ubiquitin antibody is available for research use only.
Localization:
Cell Surface, cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P0CG47, P0CG48, P62979, P62987, P62988