TRIM29 Antibody

ELISA: order BSA/sodium azide-free format for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the TRIM29 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant fragment (126 amino acid residues between aa 1-200) of human TRIM29 protein was used as the immunogen for the TRIM29 antibody.

This TRIM29 antibody is available for research use only.

Cytoplasmic & cell surface

Protein G affinity chromatography

Q14134