Topoisomerase II alpha Antibody / TOP2A
| Code | Size | Price |
|---|
| NSJ-V7234SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: TPM2A-2
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Description:
This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
7153
Immunogen:
Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this Topoisomerase II alpha antibody.
Limitation:
This Topoisomerase II alpha antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography