Topoisomerase II alpha Antibody / TOP2A
Code | Size | Price |
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NSJ-V3442SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: TOP2A/1362
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
Description:
This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
7153
Immunogen:
Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
Limitation:
This TOP2A antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P11388