Estrogen Inducible Protein pS2 / TFF1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7354SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7354SAF-100UG100 ug£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: TFF1/2133
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the pS2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of PFA-fixed human MCF7 cells with pS2 antibody (clone TFF1/2133, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human MCF7 cells with pS2 antibody (clone TFF1/2133, green) and Phalloidin (red).
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using pS2 antibody (clone TFF1/2133). These results demonstrate the foremost specificity of the TFF1/2133 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human MCF7 cells with pS2 antibody (clone TFF1/2133, green) and Reddot nuclear stain (red).
Immunofluorescent staining of PFA-fixed human MCF7 cells with pS2 antibody (clone TFF1/2133, green) and Phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using pS2 antibody (clone TFF1/2133). These results demonstrate the foremost specificity of the TFF1/2133 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the pS2 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for this pS2 antibody.
Limitation:
This pS2 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Protein G affinity chromatography
Uniprot #:
P04155