DOG1 Antibody / TMEM16A / ANO1

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NSJ Bioreagents
Product Code: NSJ-V7053IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7053IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: tDAN4
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of human gastrointestinal stromal tumor (GIST) and DOG1 antibody (clone tDAN4). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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IHC testing of human gastrointestinal stromal tumor (GIST) and DOG1 antibody (clone tDAN4). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the DOG1 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
55107
Immunogen:
Recombinant human protein was used as the immunogen for this DOG1 antibody.
Limitation:
This DOG1 antibody is available for research use only.
Localization:
Cell Surface and Cytoplasmic
Purity:
Protein G affinity chromatography