Involucrin Antibody

NSJ Bioreagents
Product Code: NSJ-V2164SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2164SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SY5
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC analysis of FFPE human tonsil tissue and Involucrin antibody.~
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SDS-PAGE Analysis of Purified, BSA-Free Involucrin Antibody (clone SY5). Confirmation of Integrity and Purity of the Antibody.

IHC analysis of FFPE human tonsil tissue and Involucrin antibody.~
SDS-PAGE Analysis of Purified, BSA-Free Involucrin Antibody (clone SY5). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by digestion with trypsin Protease XXV (Sigma) at 1mg/ml PBS for 5 min at 37°C.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3713
Immunogen:
Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
Limitation:
This Involucrin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography