p27 Antibody / p27Kip1

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NSJ Bioreagents
Product Code: NSJ-V2086SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2086SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SX53G8
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone SX53G8, green) and Phalloidin (red).
2 / 5
IHC staining of FFPE human prostate carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human colon carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Flow cytometry staining of PFA-fixed human MCF7 cells with p27Kip1 antibody; Red=isotype control, Blue= p27Kip1 antibody.
5 / 5
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone SX53G8) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone SX53G8, green) and Phalloidin (red).
IHC staining of FFPE human prostate carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry staining of PFA-fixed human MCF7 cells with p27Kip1 antibody; Red=isotype control, Blue= p27Kip1 antibody.
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone SX53G8) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
1027
Immunogen:
Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Limitation:
This p27Kip1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography