Glycophorin A Antibody

NSJ Bioreagents
Product Code: NSJ-V2563SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2563SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM599
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the anti-Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human placenta stained with anti-Glycophorin A antibody (clone SPM599). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
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IHC testing of FFPE human angiosarcoma stained with anti-Glyc

IHC testing of FFPE human placenta stained with anti-Glycophorin A antibody (clone SPM599). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
IHC testing of FFPE human angiosarcoma stained with anti-Glyc

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the anti-Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human GPA protein was used as the immunogen for the anti-Glycophorin A antibody.
Limitation:
This anti-Glycophorin A antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Uniprot #:
P02724