KRT19 Antibody / Cytokeratin 19

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NSJ Bioreagents
Product Code: NSJ-V9127SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9127SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM561
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561, green) and Reddot nuclear stain (red).
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IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (clone SPM561).
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Western blot testing of human MCF7 cells with Cytokeratin 19 antibody (clone SPM561). Predicted molecular weight ~43 kDa.
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Flow cytometry testing of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561); Red=isotype control, Blue= Cytokeratin 19 antibody.
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Immunofluorescent staining of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561, green) and Reddot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (clone SPM561).
Western blot testing of human MCF7 cells with Cytokeratin 19 antibody (clone SPM561). Predicted molecular weight ~43 kDa.
Flow cytometry testing of MeOH fixed human MCF7 cells with Cytokeratin 19 antibody (clone SPM561); Red=isotype control, Blue= Cytokeratin 19 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cytokeratin-19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human mammary epithelial organoids were used as the immunogen for this Cytokeratin 19 antibody.
Limitation:
This Cytokeratin 19 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08727