Bcl-2 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V9074SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9074SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM530
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the anti-Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC analysis of formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with anti-Bcl-2 antibody (clone SPM530).
2 / 3
Western blot of testing of human skin lysate (left) and human MCF7 cell lysate (right) using anti-Bcl-2 antibody (clone SPM530).
3 / 3
FACS staining (intracellular) of Jurkat cells using anit-Bcl-2 antibody (red) and isotype control (green).
❮❯

IHC analysis of formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with anti-Bcl-2 antibody (clone SPM530).
Western blot of testing of human skin lysate (left) and human MCF7 cell lysate (right) using anti-Bcl-2 antibody (clone SPM530).
FACS staining (intracellular) of Jurkat cells using anit-Bcl-2 antibody (red) and isotype control (green).

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for this anti-Bcl-2 antibody.
Limitation:
This anti-Bcl-2 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P10415