Mammaglobin A Antibody / SCGB2A2

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NSJ Bioreagents
Product Code: NSJ-V7951SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7951SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM518
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Mammaglobin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human samples with Mammaglobin antibody (clone SPM518). Expected molecular weight: 10-21 kDa depending on level of glycosylation.
2 / 3
IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (clone SPM518). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone SPM518) as confirmation of integrity and purity.
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Western blot testing of human samples with Mammaglobin antibody (clone SPM518). Expected molecular weight: 10-21 kDa depending on level of glycosylation.
IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (clone SPM518). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone SPM518) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Mammaglobin is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues.Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the Mammaglobin antibody.
Limitation:
This Mammaglobin antibody is available for research use only.
Localization:
Cytoplasmic, cell surface, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q13296