GFAP Antibody / Glial Fibrillary Acidic Protein

NSJ Bioreagents
Product Code: NSJ-V9023SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9023SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM507
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).~
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Western blot testing of human brain lysate GFAP antibody (clone SPM507). Predicted molecular weight ~50 kDa.

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).~
Western blot testing of human brain lysate GFAP antibody (clone SPM507). Predicted molecular weight ~50 kDa.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
GFAP isolated from pig spinal cord was used as the immunogen for this GFAP antibody.
Limitation:
This GFAP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P14136