NSJ Bioreagents

CD43 Antibody

Product Code:
 
NSJ-V9081SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM503
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Immunofluorescence staining of human K562 cells with CD43 antibody (green, clone SPM503) and Nucspot nuclear stain (red).
2 / 4
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD43 antibody (clone SPM503).
3 / 4
Western blot testing of human K562 cell lysate with CD43 antibody (clone SPM503). Expected molecular weight: 45-135 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of human PBM cells with CD43 antibody (clone SPM503); Green=isotype control, Red= CD43 antibody.
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Immunofluorescence staining of human K562 cells with CD43 antibody (green, clone SPM503) and Nucspot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD43 antibody (clone SPM503).
Western blot testing of human K562 cell lysate with CD43 antibody (clone SPM503). Expected molecular weight: 45-135 kDa depending on glycosylation level.
Flow cytometry testing of human PBM cells with CD43 antibody (clone SPM503); Green=isotype control, Red= CD43 antibody.

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NSJ-V9081SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Myeloblastic KG1 cells were used as the immunogen for this CD43 antibody.
Limitation:
This CD43 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P16150

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