PCNA Antibody

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NSJ Bioreagents
Product Code: NSJ-V9064SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9064SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM350
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (clone SPM350).~
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Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysates with anti-PCNA antibody (clone SPM350). Predicted mole
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IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (clone SPM350).~
Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysates with anti-PCNA antibody (clone SPM350). Predicted mole

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
Limitation:
This anti-PCNA antibody is available for research use only.
Localization:
Predominantly nuclear, some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P12004