Bcl-X Antibody
| Code | Size | Price |
|---|
| NSJ-V2836SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM337
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Bcl-X antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A synthetic peptide, aa 3-14 (c-QSNRELVVDFLS) from the human protein was used as the immunogen for the Bcl-X antibody.
Limitation:
This Bcl-X antibody is available for research use only.
Localization:
Cytoplasmic, cell and nuclear membrane
Purity:
Protein G affinity chromatography
Uniprot #:
Q07817