Tenascin C Antibody

NSJ Bioreagents
Product Code: NSJ-V2603SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2603SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM319
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the anti-Tenascin C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with anti-Tenascin C antibody (clone SPM319) at 4ug/ml.~
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SDS-PAGE analysis of purified, BSA-free anti-Tenascin C antibody (clone SPM319) as confirmation of integrity and

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with anti-Tenascin C antibody (clone SPM319) at 4ug/ml.~
SDS-PAGE analysis of purified, BSA-free anti-Tenascin C antibody (clone SPM319) as confirmation of integrity and

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the anti-Tenascin C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human breast carcinoma cells were used as the immunogen for the anti-Tenascin C antibody.
Limitation:
This anti-Tenascin C antibody is available for research use only.
Localization:
Connective tissue matrix
Purity:
Protein G affinity chromatography
Uniprot #:
P24821