Plasma Cell Marker Antibody

Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Optimal dilution of the antibody should be determined by the researcher.

It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This mAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Pancreatic cancer-related mucin was used as the immunogen for this Plasma Cell Marker antibody.

This Plasma Cell Marker antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography