HSP27 Antibody / HSPB1

Contact us
NSJ Bioreagents
Product Code: NSJ-V9024SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9024SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM252
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 6
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
2 / 6
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
3 / 6
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252, green) and Reddot nuclear stain (red).
4 / 6
Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252); Red=isotype control, Blue= HSP27 antibody.
5 / 6
Western blot testing of human HeLa cell lysate with HSP27 antibody (clone SPM252). Expected molecular weight: 24-29 kDa.
6 / 6
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.
❮❯

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252); Red=isotype control, Blue= HSP27 antibody.
Western blot testing of human HeLa cell lysate with HSP27 antibody (clone SPM252). Expected molecular weight: 24-29 kDa.
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
Limitation:
This HSP27 antibody is available for research use only.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P04792