GRP94 Antibody / HSP90B1
Code | Size | Price |
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NSJ-V2909IHC-7ML | 7 ml | £534.00 |
Quantity:
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Overview
Host Type: Rat
Antibody Isotype: Rat IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM249
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the gp96 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the gp96 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.
Limitation:
This gp96 antibody is available for research use only.
Localization:
Cytoplasmic and nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P14625