GFAP Antibody / Glial Fibrillary Acidic Protein

NSJ Bioreagents
Product Code: NSJ-V9022SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9022SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM248
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).
2 / 4
Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.
3 / 4
Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.
4 / 4
SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.

IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).
Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.
Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.
SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.
Limitation:
This Glial Fibrillary Acidic Protein antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P14136