Bromodeoxyuridine / BrdU Antibody

NSJ Bioreagents
Product Code: NSJ-V9115IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9115IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM166
Regulatory Status: RUO
Target Species: Species Independent
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the anti-Bromodeoxyuridine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded mouse intestine stained with anti-Bromodeoxyuridine antibody (SPM166).

IHC: Formalin-fixed, paraffin-embedded mouse intestine stained with anti-Bromodeoxyuridine antibody (SPM166).

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Bromodeoxyuridine was used as the immunogen for this anti-Bromodeoxyuridine antibody.
Limitation:
This anti-Bromodeoxyuridine antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography