PMEL17 / Melanoma gp100 Antibody

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the antibody should be determined by the researcher.

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma.

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.

This gp100 antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

P40967