HCG-beta Antibody

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NSJ Bioreagents
Product Code: NSJ-V3708SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3708SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM105
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HCG-beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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IHC testing of FFPE human placenta with hCG beta antibody (clone SPM105). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human placenta with hCG beta antibody (clone SPM105). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the HCG-beta antibody for each application should be determined by the researcher.

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. hCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant hCG beta protein was used as the immunogen for this hCG beta antibody.
Limitation:
This HCG-beta antibody is available for research use only.
Localization:
Cytoplasmic, Secreted
Purity:
Protein G affinity chromatography
Uniprot #:
P01233